Journal: Journal of Animal Science and Biotechnology
Article Title: High expression circRALGPS2 in atretic follicle induces chicken granulosa cell apoptosis and autophagy via encoding a new protein
doi: 10.1186/s40104-024-01003-w
Figure Lengend Snippet: CircRNA RNC-seq analysis of healthy and atretic follicles. A Schematic sequencing of healthy and atretic follicles. B Volcano plot of differentially expressed circRNAs in circRNA RNC-seq. C Volcano plot of differentially expressed circRNAs in circRNA RNA-seq. D Combined analysis of circRNA RNA-seq and RNC-seq. E Heatmap of 38 differentially expressed circRNAs in circRNA RNA-seq and RNC-seq. F Details of the top 5 circRNAs with the highest expression derived from exon splicing. G qRT-PCR validation of five dierentially expressed circRNA in healthy and atretic follicles. * P < 0.05, ** P < 0.01. Data are presented as mean ± SEM. n = 9 for qRT-PCR
Article Snippet: A circRNA RNC-seq analysis was performed by Gene Denovo Biotechnology Co., Ltd. (Guangzhou, Guangdong, China).
Techniques: Sequencing, RNA Sequencing, Expressing, Derivative Assay, Quantitative RT-PCR, Biomarker Discovery